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KMID : 1234420090370010055
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Korean Journal of Microbiololgy and Biotechnology
2009 Volume.37 No. 1 p.55 ~ p.61
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Conversion of Ginsenoside Rb1 by Ginseng Soil Bacterium Cellulosimicrobium sp. Gsoil 235 According to Various Culture Broths
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Na Ju-Ryun
Kim Yu-Jin
Kim Se-Hwa
Kim Ho-Bin
Shim Ju-Sun
Kim Se-Young
Yang Deok-Chun
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Abstract
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Ginseng saponins (a secondary metabolite, termed ginsenosides) are the principal bioactive ingredients of ginseng, and modification of the sugar chains may markedly change the its biological activity. One of soil bacteria having β-glucosidase (to transform ginsenoside Rb1) activity was isolated from soil of a ginseng field in Daejeon. 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Cellulosimicrobium, with highest sequence similarity (99.7%) to Cellulosimicrobium funkei ATCC BAA-886T. The strain, Gsoil 235, could transform ginsenoside Rb1 into Rd, Rg3 and 3 of un-known ginsenosides by the analyses of TLC, HPLC. By investigating its deglycosylation progress, the optimal broth for β-glucosidase was nutrient broth (In 48 hours, almost ginsenoside Rb1 could be transformed into minor ginsenosides). On the contrary, the optimal broth for growth was determined as trypic soy broth (TSB).
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KEYWORD
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Panax ginseng C. A, Meyer, Cellulosimicrobium sp., conversion, ginsenoside Rb1, ginsenoside Rg3, TLC, HPLC
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